ABSTRACT
The aim of thos survey is establishment of an animal model through which a rapid distortion of the hair cell of cochlea administering amikacin and furosemide and using distortion product otoacoustic emission [DPOAE] the functioning of rat's ear would be assessed. Forty-eight male Sprague dawley rats [with average of weight 200-250g, 12 weeks post natal] were randomly divided into six equal groups. Except the control group the rest received 0.5mg/g, 0.75mg/g, 1mg/g, 1.25mg/g, and 1.5mg/g, of subcutaneous amikacin respectively. 30 minutes later every rat received 0.1mg/g of furosemide intrapritoneally. DPOAE of rats was measured prior to these injections and 72 hours later. Then tissue section of the rat's cochlea was prepared.Data were analyzed by means of SPSS [version 17] and ANOVA test and paired sample T test at the significant level of p